                                     mse



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Function

   Multiple sequence editor

Description

   This is a port of Will Gilbert's MSE multiple sequence editor from the
   original C code.

Algorithm

Usage

   Here is a sample session with mse


% mse dna.msf msf::mse.msf
Multiple sequence editor


   Go to the input files for this example
   Go to the output files for this example

Command line arguments

Multiple sequence editor
Version: EMBOSS:6.6.0.0

   Standard (Mandatory) qualifiers:
  [-sequence]          seqset     File containing a sequence alignment
  [-outseq]            seqoutset  [.] Sequence set filename
                                  and optional format (output USA)

   Additional (Optional) qualifiers: (none)
   Advanced (Unprompted) qualifiers:
   -gcgmode            boolean    [N] Use GCG Unix mode for keyboard shortcuts
                                  and commands
   -protein            boolean    [$(acdprotein)] Sequences are protein

   Associated qualifiers:

   "-sequence" associated qualifiers
   -sbegin1            integer    Start of each sequence to be used
   -send1              integer    End of each sequence to be used
   -sreverse1          boolean    Reverse (if DNA)
   -sask1              boolean    Ask for begin/end/reverse
   -snucleotide1       boolean    Sequence is nucleotide
   -sprotein1          boolean    Sequence is protein
   -slower1            boolean    Make lower case
   -supper1            boolean    Make upper case
   -scircular1         boolean    Sequence is circular
   -squick1            boolean    Read id and sequence only
   -sformat1           string     Input sequence format
   -iquery1            string     Input query fields or ID list
   -ioffset1           integer    Input start position offset
   -sdbname1           string     Database name
   -sid1               string     Entryname
   -ufo1               string     UFO features
   -fformat1           string     Features format
   -fopenfile1         string     Features file name

   "-outseq" associated qualifiers
   -osformat2          string     Output seq format
   -osextension2       string     File name extension
   -osname2            string     Base file name
   -osdirectory2       string     Output directory
   -osdbname2          string     Database name to add
   -ossingle2          boolean    Separate file for each entry
   -oufo2              string     UFO features
   -offormat2          string     Features format
   -ofname2            string     Features file name
   -ofdirectory2       string     Output directory

   General qualifiers:
   -auto               boolean    Turn off prompts
   -stdout             boolean    Write first file to standard output
   -filter             boolean    Read first file from standard input, write
                                  first file to standard output
   -options            boolean    Prompt for standard and additional values
   -debug              boolean    Write debug output to program.dbg
   -verbose            boolean    Report some/full command line options
   -help               boolean    Report command line options and exit. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning            boolean    Report warnings
   -error              boolean    Report errors
   -fatal              boolean    Report fatal errors
   -die                boolean    Report dying program messages
   -version            boolean    Report version number and exit


Input file format

   mse reads any normal sequence USAs.

  Input files for usage example

  File: dna.msf

!!NA_MULTIPLE_ALIGNMENT

 dna.msf  MSF: 120  Type: N  January 01, 1776  12:00  Check: 3196 ..

 Name: MSFM1          Len:   120  Check:  8587  Weight:  1.00
 Name: MSFM2          Len:   120  Check:  6178  Weight:  1.00
 Name: MSFM3          Len:   120  Check:  8431  Weight:  1.00

//

        MSFM1  ACGTACGTAC GTACGTACGT ACGTACGTAC GTACGTACGT ACGTACGTAC
        MSFM2  ACGTACGTAC GTACGTACGT ....ACGTAC GTACGTACGT ACGTACGTAC
        MSFM3  ACGTACGTAC GTACGTACGT ACGTACGTAC GTACGTACGT CGTACGTACG

        MSFM1  GTACGTACGT ACGTACGTAC GTACGTACGT ACGTACGTAC GTACGTACGT
        MSFM2  GTACGTACGT ACGTACGTAC GTACGTACGT ACGTACGTAC GTACGTACGT
        MSFM3  TACGTACGTA CGTACGTACG TACGTACGTA ACGTACGTAC GTACGTACGT

        MSFM1  ACGTACGTAC GTACGTACGT
        MSFM2  ACGTACGTTG CAACGTACGT
        MSFM3  ACGTACGTAC GTACGTACGT


Output file format

   mse outputs a graph to the specified graphics device. outputs a report
   format file. The default format is ...

  Output files for usage example

  File: mse.msf

!!NA_MULTIPLE_ALIGNMENT 1.0

  mse.msf MSF: 120 Type: N 15/07/10 CompCheck: 2784 ..

  Name: MSFM1      Len: 120  Check: 8587 Weight: 1.00
  Name: MSFM2      Len: 120  Check: 5766 Weight: 1.00
  Name: MSFM3      Len: 120  Check: 8431 Weight: 1.00

//

           1                                               50
MSFM1      ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTAC
MSFM2      ACGTACGTACGTACGTACGTacgt....ACGTACGTACGTACGTACGTAC
MSFM3      ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTCGTACGTACG

           51                                             100
MSFM1      GTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGT
MSFM2      GTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGT
MSFM3      TACGTACGTACGTACGTACGTACGTACGTAACGTACGTACGTACGTACGT

           101              120
MSFM1      ACGTACGTACGTACGTACGT
MSFM2      ACGTACGTTGCAACGTACGT
MSFM3      ACGTACGTACGTACGTACGT


Data files

   None.

Notes

   None.

References

   None.

Warnings

   None.

Diagnostic Error Messages

   None.

Exit status

   It always exits with status 0.

Known bugs

   None.

See also

   Program name     Description
   edialign         Local multiple alignment of sequences
   emma             Multiple sequence alignment (ClustalW wrapper)
   eomega           Multiple sequence alignment (ClustalO wrapper)
   eomegapp         Profile with profile (ClustalO wrapper)
   eomegaps         Single sequence with profile (ClustalO wrapper)
   eomegash         Sequence with HMM (ClustalO wrapper)
   eomegasp         Sequence with profile (ClustalO wrapper)
   infoalign        Display basic information about a multiple sequence alignment
   plotcon          Plot conservation of a sequence alignment
   prettyplot       Draw a sequence alignment with pretty formatting
   showalign        Display a multiple sequence alignment in pretty format
   tranalign        Generate an alignment of nucleic coding regions from aligned
                    proteins

Author(s)

   This program is an EMBOSS conversion of a program written by Will
   Gilbert as part of his MSE package.

   Please report all bugs to the EMBOSS bug team
   (emboss-bug (c) emboss.open-bio.org) not to the original author.

History

Target users

   This program is intended to be used by everyone and everything, from
   naive users to embedded scripts.

Comments

None
